一、首发文献
He, Y., Zhang, T., Sun, H. et al. A reporter for noninvasively monitoring gene expression and plant transformation. Hortic Res 7, 152 (2020). https://doi.org/10.1038/s41438-020-00390-1
二、Ruby基因序列
烟草和拟南芥的2A肽序列不太一样，如何设计2A肽？
We took advantage of the P2A peptide, which has the sequence of (GSGATNFSLLKQAGDVEENPGP), to link the CYP76AD1, DODA, and glucosyltransferase (GT) coding regions. The entire sequence of RUBY and terminator was shown in Supplemental Fig. 1.
The three genes were linked by sequences (red) that encode 2A peptides. Note that the two 2A sequences differed in D
